five M NaCl, pH eight.0); strip buffer (0.1 M Ethylenediaminetetraacetic acid (EDTA)), 0.5 M NaCl
five M NaCl, pH eight.0); strip buffer (0.1 M Ethylenediaminetetraacetic acid (EDTA)), 0.5 M NaCl, pH eight.0). For P450cam purifications, all buffers contained 1 mM camphor. Substratefree P450 was ready…