Product Name :
PNKP Recombinant Rabbit Monoclonal Antibody [JE64-85]
Predicted band size :
57 kDa
Observed band size :
60 kDa
Synonyms:
2”(3”)-polynucleotidase antibody 2′(3′)-polynucleotidase antibody Bifunctional polynucleotide phosphatase/kinase antibody DEM 1 antibody DEM1 antibody DNA 5′ kinase/3′ phosphatase antibody DNA 5”-kinase/3”-phosphatase antibody EIEE10 antibody Homo sapiens polynucleotide kinase 3′ phosphatase (PNKP) antibody MCSZ antibody 展开
Function :
Bifunctional polynucleotide phosphatase/kinase is an enzyme that in humans is encoded by the PNKP gene. A detailed structural study of the crystallized mouse protein examined both the 5´-polynucleotide kinase and 3’-polynucleotide phosphatase activities. Additional features of the peptide sequence include a forkhead association (FHA) domain, ATP binding site and nuclear and mitochondrial localization sequences. PNKP has been shown to interact with DNA polymerase beta and XRCC1. The human gene encoding PNKP was observed to be mutated in patients with microcephaly, seizures and defects in DNA repair. A type of recessive ataxia is also associated with PNKP mutations. There are also newly characterized pathological variants of PNKP. Model organisms such as mice and Drosophila have been used to generate further insights.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic peptide within human PNKP aa 460-509/521.5-Hydroxymethylfurfural web
Species reactivity:
Human
Verify application:
WB, IHC-P
Coupling :
unconjugated
Clone number:
JE64-85
RRID:
AB_3072211
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.Formula of 2832911-62-1 Aliquot store at -20℃.PMID:33590403 Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 57 kDa
Positive control :
HepG2 cell lysate, 293 cell lysate, PC-3 cell lysate, Jurkat cell lysate, human colon carcinoma tissue.